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(A) Heatmaps reporting the level (low: blue, average: white, high: red) of protein expression across samples for a selection of morphogens, growth factors and cognate receptors. The heatmap was generated using Morpheus (the Broad Institute, MIT). ‘Not a Number’ (NaN) values are in grey. (B) Heatmap of protein expression for cortical layer markers. (C) Heatmap of protein expression for chemoattractive/chemorepulsive molecules. (D) Proteins known to be expressed in neural progenitors, neurons, astrocytes and oligodendrocytes are represented as concentric circles proportional to their relative abundance (iBAQ) in D21 organoids. (E) D21 organoid cryosections were stained with <t>REELIN</t> <t>(green),</t> <t>GRM5</t> (orange), and DAPI (blue) and imaged at a fluorescent microscope using the apotome mode. Scale bar: 200 µm. (F) High magnification images from the inset drawn in E. Scale bars: 10 µm.
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Image Search Results


(A) Heatmaps reporting the level (low: blue, average: white, high: red) of protein expression across samples for a selection of morphogens, growth factors and cognate receptors. The heatmap was generated using Morpheus (the Broad Institute, MIT). ‘Not a Number’ (NaN) values are in grey. (B) Heatmap of protein expression for cortical layer markers. (C) Heatmap of protein expression for chemoattractive/chemorepulsive molecules. (D) Proteins known to be expressed in neural progenitors, neurons, astrocytes and oligodendrocytes are represented as concentric circles proportional to their relative abundance (iBAQ) in D21 organoids. (E) D21 organoid cryosections were stained with REELIN (green), GRM5 (orange), and DAPI (blue) and imaged at a fluorescent microscope using the apotome mode. Scale bar: 200 µm. (F) High magnification images from the inset drawn in E. Scale bars: 10 µm.

Journal: bioRxiv

Article Title: The RNA and protein landscapes of mouse brain organoids

doi: 10.64898/2026.03.13.711293

Figure Lengend Snippet: (A) Heatmaps reporting the level (low: blue, average: white, high: red) of protein expression across samples for a selection of morphogens, growth factors and cognate receptors. The heatmap was generated using Morpheus (the Broad Institute, MIT). ‘Not a Number’ (NaN) values are in grey. (B) Heatmap of protein expression for cortical layer markers. (C) Heatmap of protein expression for chemoattractive/chemorepulsive molecules. (D) Proteins known to be expressed in neural progenitors, neurons, astrocytes and oligodendrocytes are represented as concentric circles proportional to their relative abundance (iBAQ) in D21 organoids. (E) D21 organoid cryosections were stained with REELIN (green), GRM5 (orange), and DAPI (blue) and imaged at a fluorescent microscope using the apotome mode. Scale bar: 200 µm. (F) High magnification images from the inset drawn in E. Scale bars: 10 µm.

Article Snippet: The primary antibodies were (species, provider; catalog number): anti-NESTIN –Rat-401- (mouse, Santa-Cruz, sc-33677); PAX6 (Rabbit, Covance, PRB-278P); NANOG (mouse, BD Pharmingen, 560259); POU5F1 (rabbit, Cell Signalling, 2840), ELAVL3 (mouse, Santa Cruz, sc-515624), TUBB3 (mouse, Covance, MMS-435P); TBR1 (rabbit, Cell Signalling, 49661), REELIN (mouse, Covance; MAB5364), GRM5 (rabbit, Millipore, AB5675), and GFAP (rabbit, Dako, Z0334).

Techniques: Expressing, Selection, Generated, Staining, Microscopy